Mots-Clés

genomic imprinting long reads sequencing technologies pig methylation reciprocal crosses

Description

You will be welcomed in the Genetics, Physiology and Livestock Systems Laboratory (GenPhySE) in the Molecular Genetics and Epigenetics team (GenEpi, https://genphyse.toulouse.inra.fr/groups/genepi?page=2) located at the INRAE Toulouse-Occitania campus. You will work as part of the ANR PIPETTE and FEDER SeqOccIN projects.

These projects will have (i) scientific outputs via the characterization of the first imprinting map in the pig species and (ii) technological outputs via the evaluation of 2 epi-genotyping methods targeted to imprinted genes. In addition to shedding new light on the evolution of parental imprinting mechanisms, your research will provide a generic approach to this original molecular process for the study of birth weight variability in piglets.

Within the framework of these projects, multi-omics data (transcriptomic, genomic and epigenomic) have been produced, via long-read sequencing technologies (ONT and PacBio) or targeted region sequencing (Illumina capture), for individuals from reciprocal crosses. By analyzing this large dataset produced from an original experimental design, you will characterize, for the first time at the genome scale, the molecular signatures of parental imprinting genes in pigs.

• Use appropriate bioinformatics tools to analyze the different transcriptomic, genomic and epigenomic sequencing data from several sequencing technologies. ·
• Identify, via classical statistical analyses, genes showing parental imprinting in different tissues by leveraging genetic, expression and cross-over information. ·
• Characterize the molecular signature of the identified genes by determining the parental origin of the expressed and/or methylated alleles and thus compare inter-tissue and even inter-species variability.
• Engage in an open science approach with genericity and availability of scripts and code necessary for the analyses.
• Write and publish articles in peer-reviewed journals.