Mots-Clés

Single cell RNA sequencing, multiomics, stem cell, musculoskeletal regeneration,

Description

POST-DOCTORAL POSITION IN BIOINFORMATICS ANALYSES

INSERM, Mondor Biomedical Research Institute, Univ Paris-Est Créteil, France

Colnot group “Stem cells in musculoskeletal development, regeneration and diseases” colnotlab.com

We are seeking a highly motivated and enthusiastic postdoctoral candidate to join our team to study skeletal stem/progenitor cells (SSPCs) in bone regeneration. SSPCs comprise a diversity of cell populations that are essential for bone repair. The laboratory has shown that SSPCs reside in several bone compartments including the bone marrow and the periosteum (the tissue at the outer surface of bone), but also in adjacent skeletal muscle. In response to bone injury, SSPCs sense signals in their local tissue environment, differentiate and must self-renew. Disruption of SSPC niche can lead to delayed or failed healing. Our goal is to better understand the diversity of SSPCs, the SSPC-niche interactions and how SSPCs are affected in bone repair disorders, to explore new therapeutic strategies for bone regeneration. We examine the cellular and molecular bases of fracture non-union in genetically modified mouse models and patient samples to uncover the mechanisms of skeletal stem cell dysfunctions during bone repair. We also use single cell transcriptomics and multiomics analyses in parallel with functional analyses.

Project: Study the diversity and regenerative potentials of SSPCs in bone repair and in disease conditions, using RNAseq analyses, single cell RNAseq analyses and multiomics analyses. Large-scale transcriptome analyses will be based on data generated in the laboratory to investigate the diversity, spatial relationship and paracrine interactions between SSPCs and other cell types. Candidates should be familiar with programming in languages including R and Python and have good knowledge in cell biology, gene expression and regulation.

The applicants should be PhDs. Ability to initiate, conduct, evaluate and report research studies in English is essential. Salary is available for 3 years, renewed on a yearly basis according to progress. In addition to completing this funded research, the candidate will be strongly encouraged to develop research objectives that will lead to fellowship funding. 

Mondor Biomedical Research Institute is a large research institute located in the east of Paris. The institute is affiliated with Henri Mondor Hospital, the faculty of Health and Université Paris-Est Créteil, providing a multidisciplinary research environment and state-of-the-art research platforms.

Recent publications from the Colnot Group:

1. Julien, A., Perrin, S., Martínez‐Sarrà, E., Kanagalingam, A., Carvalho, C., Luka, M., Ménager, M., & Colnot, C. (2022). Skeletal stem/progenitor cells in periosteum and skeletal muscle share a common molecular response to bone injury. Journal of Bone and Mineral Research, 37(8), 1545-1561. https://doi.org/10.1002/jbmr.4616
2. Perrin, S., & Colnot, C. (2022). Periosteal skeletal stem and progenitor cells in bone regeneration. Current Osteoporosis Reports, 20(5), 334-343. https://doi.org/10.1007/s11914-022-00737-8
3. Julien, A., Kanagalingam, A., Martinez Sarra, E., Megret, J., Luka M., Menager, M., Relaix, F., Colnot, C. (2021). Direct contribution of skeletal muscle mesenchymal progenitors to bone repair. Nature Communications, 12(1): 2860. https://doi.org/10.1038/s41467-021-22842-5
4. Julien, A., Perrin, S., Duchamp de Lageneste, O., Carvalho, C., Bensidhoum, M., Legeai-Mallet, L., & Colnot, C. (2020). Fgfr3 in periosteal cells drives cartilage-to-bone transformation in bone repair. Stem Cell Reports, 15(4), 955-967. https://doi.org/10.1016/j.stemcr.2020.08.005
5. Duchamp de Lageneste, O, Julien, A, Abou-Khalil, R, Frangi, G, Carvalho, C, Cagnard, N, Cordier, C, Conway, SJ and Colnot, C. Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin. Nature Communications, 2018 Feb 22;9(1):773. Rai M and Duan X: F1000Prime Recommendation, 22 May 2018. DOI: 10.1038/s41467-018-03124-z