Mots-Clés

DNA repilcation/repair Genome Stability NGS Nanopore sequencing High-throughput single-molecule imaging Bionano

Description

Bioinformatician - Postdoc/IR (M/F)

The hosting structure
Institut Curie Research Center
Institut Curie is a major player in the research and fight against cancer. It consists of a hospital group and a Research Center
of more than 1000 employees with a strong international representativeness.
The objective of the Research Center is to develop basic research and to use the knowledge produced to improve the
diagnosis, prognosis, and therapeutics of cancers as part of the continuum between basic research and innovation serving
the patient.

Context
Laboratory
We are seeking a motivated and productive bioinformatician (M/F) to join our team “Replication Program and Genome
Instability” (Team CHEN) at the Institut Curie, Paris, France. The team focuses on using cutting-edge high-throughput
genomic approaches and genome-wide data analyses to study the spatio-temporal replication program of the human
genome and how replication stress impacts on genome stability in normal and cancer cells, in population as well as at single
molecule/cell level.
The project
The successful candidate (M/F) will join our interdisciplinary effort to uncover the mechanisms safeguarding genome
stability during DNA replication and repair. DNA double-strand breaks (DSBs) and replication stress are critical threats to
genome integrity, linked to diseases like cancer and neurological disorders. Our projects integrate cutting-edge highthroughput
single-molecule imaging (e.g. Bionano) and sequencing (e.g. Nanopore) with computational modeling and
bioinformatics to study these processes at unprecedented resolution. The interplay between DNA replication and repair is
crucial for maintaining genome integrity. Emerson et al. (2022, Nature) highlighted how replication forks can stall and
collapse into DSBs, necessitating homologous recombination (HR)-mediated repair, while Li et al. (2023, Cell) described how
replication initiates and how replication stress triggers specific signaling cascades to protect genome stability. Our
integrated projects aim to map how replication stress induces repair pathways and how misregulation contributes to disease
progression.
The ANR “SMART” project focuses on homologous recombination (HR), a key pathway for DSB repair, exploring its
regulation and interplay with chromatin structure and spatial genome organization. Meanwhile, the “Impulscience” project
investigates DNA replication programs, their adaptation during development, and their deregulation in pathological
conditions such as cancer. By leveraging innovative technologies and collaborating with leading experts, we aim to unravel
how replication stress and DNA repair intersect to maintain genome stability.
By combining insights from these landmark studies with novel high-throughput approaches, our projects will illuminate
how the replication and repair machinery coordinates to preserve genome stability. This interdisciplinary effort is expected
to yield transformative insights into the molecular underpinnings of diseases like cancer and neurodegenerative disorders.
Join our team to contribute to transformative research at the interface of molecular biology, genomics, and bioinformatics,
paving the way for new insights into genome maintenance and its role in human disease.

Bibliographical references
1. Arnould, C., Nature, 590, 660-665 (2021).
2. Wang, W., Mol. Cell, 81, 2975-2988.e6 (2021)
3. Promonet, A., Nat. Commun. 11:3940 (2020)
4. Gnan, S., Nat. Commun., 13, 2329 (2022)
5. Vernekar, DV., Nucleic Acids. Res., 49, 4522-4533. (2021)
6. Emerson, Nature. 606:812–819 (2022)
7. Li, J., Cell, 186:98-111.e21 (2023)
8. Liu, Y., et al. Nucleic Acids. Res., gkac1239 (2023)

Constraints and risks
- Occasional travel: to attend project meetings, workshops, or conferences with collaborators.
- Prolonged sitting: Ergonomic accommodation can be arranged to mitigate discomfort.

Candidate Profile
Training and Skills required:
- Applicant (M/F) should hold, or in the process of completing, a PhD degree in biology, bioinformatics, or related
areas.
- Scientific skills:
o Solid computational/statistical skills,
o Solid background in molecule/cellular biology,
o Strong interest in genome biology.
- Experience:
o High-throughput sequencing data analysis,
o 3rd generation sequencing (Nanopore sequencing),
o Lineage tracing analysis is a plus,
o High-throughput imaging analysis is a plus.
- Language skills: very good English level (working language) and communication skills.

Abilities
- Ability to work independently, managerial abilities, aptitude for working in a team.
- The candidate (M/F) should be highly motivated, curious and enthusiastic to work in a collaborative team.
All our opportunities are open to people with disabilities

Contract information
Type of contract: Fixed-term contract.
Starting date: January 2025
Duration: 2 years
Working time: full time
Remuneration: according to the current grids
Benefits: Collective catering, reimbursement of transportation fees up to 70%, supplementary health insurance
Location of the position: Paris, 5eme

Contact
Please send your CV, letter of motivation and 3 references to job-ref-p2l94ee0gh@emploi.beetween.com
CVs to be processed on the go, therefore the position could be filled before the deadline for applications.

Institut Curie is an inclusive, equal opportunity employer and is dedicated to the highest standards of research integrity.
https://euraxess.ec.europa.eu/sites/default/files/brochures/eur_21620_en-fr.pdf