Mots-Clés

single cell RNA sequencing and spatial transcriptomics analysis

Description

Position
M2 internship position is available in the Guermonprez lab at Institut Pasteur Paris (“Dendritic cells and adaptive immunity” Unit, Immunology Department https://research.pasteur.fr/fr/department/immunology/).
The Guermonprez lab investigates the role of dendritic cell subsets in regulating T cell responses (https://www.guermonprezlab.org).

Project
The Guermonprez lab investigates the cellular basis of anti-tumor immunity. We intend to understand the spatial organization of immune infiltratres in the tumor micro-environment (dendritic cells and anti-tumoral T lymphocytes) and to decipher how immunotherapies impact on these processes. The main objectives of this project are:
- to implement single cell RNAseq analysis of immune cells infiltrating tumors using available datasets;
- to implement spatial transcriptomics of the tumor microenvironment (VISIUM HD, Merscope, e.g.) using available datasets.

Mission
The candidate will:

• Conduct analyses of experimental data generated in the lab (single cell RNAseq, spatial transcriptomics)
• Present results in laboratory and collaborative meetings.
• Collaborate actively with scientists performing wet bench experiments (PhD, postdocs). 
• Interact productively with other bioinformatic engineers or researchers on Institut Pasteur campus.

Profile
We are looking for highly motivated individuals motivated to bring new insights into the biology of adaptive immunity against cancer.
The M2 candidate is expected to have:

• Interest for methodologies related to the analysis of biological processes at the single cell level
• Dynamism, self-organization, autonomy and drive.
• Good interaction skills with wet bench scientists and biologists in general.
• Enthusiasm for academic research on cancer immunology and immunotherapy.